Московский Государственный Университет имени М.В.Ломоносова
Биологический факультет
Кафедра биофизики

119991, Москва, ГСП-2, Ленинские горы. Телефон (495) 939-1116, факс 939-1115.
!Поздравляем старшего научного сотрудника кафедры биофизики Надежду Александровну Браже с победой в конкурсе «For Women in Science» и премией ЮНЕСКО за 2015 год!

Лаборатория общей биофизики

Сёмин Борис Константинович – д.б.н., доцент, вед.н.сотр

Гуськова Р.А., Давлетшина Л.Н., Ловягина Е.Р., Локтюшкин А.В., Федоров Г.Е.

Основные направления научной работы

Конкретные задачи, решаемые в рамках фундаментальных направлений исследований 

Методы исследований 

Научные контакты 

 Учебная работа 

 

Студенты и аспиранты


Избранные публикации


Semin B.K. Seibert M. (2016) Substituting Fe for two of the four Mn ions in photosystem II—effects on water-oxidation. J Bioenerg Biomembr 48(3) 227-240 DOI 10.1007/s10863-016-9651-2

 

Lovyagina E.R., Semin B.K. (2016) Mechanism of inhibition and decoupling of oxygen evolution from electron transfer in photosystem II by fluoride, ammonia and acetate. Journal of Photochemistry & Photobiology, B: Biology 158:145–153

 

Boris K. Semin & Tatiana E. Podkovirina & Lira N. Davletshina & Kirill N. Timofeev & Il’ya I. Ivanov & Andrei B. Rubin  The extrinsic PsbO protein modulates the oxidation/reduction rate of the exogenous Mn cation at the high-affinity Mn-binding site of Mn-depleted PSII membranes.  J Bioenerg Biomembr (2015) 47:361–367

 

Semin BK, Davletshina LN, Rubin AB (2015) Correlation between pH dependence of O2 evolution and sensitivity of Mn cations in the oxygen-evolving complex to exogenous reductants. Photosynth Res. 125:95-103

 

Boris K. Semin, Lira N. Davletshina, Kirill N. Timofeev, Il’ya I. Ivanov, Andre B. Rubin &Michael Seibert (2013) Production of reactive oxygen species indecoupled, Ca2+-depleted PSII and their use in assigning a function to chloride on both sides of PSII. Photosynthesis Res.(2013) 117, 385-399. DOI10.1007/s11120-013-9870-x 

      

Kurashov V.N., Lovyagina E.R., Shkolnikov D.Yu., Solntsev M.K.,   Mamedov M.D., Semin B.K. (2009) Investigation of the low-affinity oxidation site for exogenous electron donors in the Mn-depleted photosystem II complexes.  Biochim Biophys Acta 1787, 1492-1498.

 

Semin B.K. and Seibert M. A (2009) Simple Colorimetric Determination of theManganese Content in Photosynthetic Membranes. Photosynth. Res. 100, 45-48.

 

Makarova, М., S. Kosourov, T. Krendeleva, B. Semin, G. Kukarskikh, A. Rubin, R.Sayre, M. Ghirardi, and M. Seibert. Photoproduction of hydrogen by sulfur-deprived C. reinhardtii mutants with impaired Photosystem II photochemical activity. Photosynth.Res. (2007) 94, 79-89.

 

Semin B.K., Davletshina L.N., Ivanov I.I., Rubin A.B., and Seibert M. (2008)Decoupling of the processes of molecular oxygen synthesis and electron transport in Ca2+-depleted PSII membranes. Photosynth. Res. 98, 235-249.

 

Semin B.K. and Seibert M. A carboxylic residue at the high-affinity, Mn-bindingsite participates in the binding of iron cations that block the site. Biochim. Biophys. Acta 2006, 1757, 189-197.

 

Semin B.K. and Seibert M. Flash-Induced Blocking of the High-Affinity Manganese-Binding Site in Photosystem II by Iron Cations: Dependence on the Dark Interval between Flashes and Binary Oscillations of Fluorescence Yield. J. Phys. Chem. B 2006, 110, 25532-25542.

 

Semin B.K, Lovyagina E.R, Timofeev K.N, Ivanov I.I, Rubin A.B, Seibert M.Iron-Blocking the High-Affinity Mn-Binding Site in Photosystem II Facilitates Identification of the Type of Hydrogen Bond Participating in Proton-Coupled Electron Transport via Y(Z)(*). Biochemistry, 2005, 44(28), 9746-9757.

 

Semin B.K. and Seibert M. Iron bound to the high-affinity Mn-binding site ofthe oxygen-evolving complex shifts the pK of a component controlling electron transport via YZ. Biochemistry, 2004, 43(21), 6772-6782.

 

Semin B.K., Davletshina L.N., Novakova A.A., Kiseleva T.I., Lanchinskaya V.Y., Aleksandrov A.Y., Seifulina N., Ivanov I.I., Seibert M., Rubin A.B. Accumulation of Ferrous Iron in Chlamidomonas reinhardtii. Influence of CO(2) and Anaerobic Induction of the Reversible Hydrogenase. Plant Physiol. (2003) 132, 1756-1764.

 

Semin, B.K., Ghirardi, M.L., Seibert, M. (2002) Blocking of electron donation by Mn(II) to Yz· following incubation of Mn-depleted photosystem II membranes with Fe(II) in the light. Biochemistry 41(N18): 5854-5864.

 

Ahlbrink, R., Semin, B.K., Mulkidjanian, A.Yu., and Junge, W. (2001) Photosystem II of pears: effect of added divalent cations of Mn, Fe, Mg and Ca on two kinetic components of P680+ reduction in Mn-depleted core particles. Biochim.Biophys.Acta 1506, 117-126.

 

Semin BK, Ivanov II, Rubin AB, Carpentier R. pH-dependent extraction of Ca2+ from photosystem II and thylakoid membranes: indication of a Ca2±sensitive site on the acceptor side of photosystem II. Photochem Photobiol. 1998, 68(4), 538-544.

 

Semin B.K.,  Parak F.  Coordination spere and structure of the Mn cluster of the oxygen-evolving complex in photosynthetic organisms// (1997)FEBS Lett. V. 400, p.259-262.